Reconstitution of yeast translation initiation

被引：121

作者：

Acker, Michael G. ^{[1
]}

Kolitz, Sarah E. ^{[1
]}

Mitchell, Sarah F. ^{[1
]}

Nanda, Jagpreet S. ^{[1
]}

Lorsch, Jon R. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA

来源：

TRANSLATION INITIATION: RECONSTITUTED SYSTEMS AND BIOPHYSICAL METHODS | 2007年 / 430卷

关键词：

D O I：

10.1016/S0076-6879(07)30006-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To facilitate the mechanistic dissection of eukaryotic translation initiation we have reconstituted the steps of this process using purified Saccharomyces cerevisiae components. This system provides a bridge between biochemical studies in vitro and powerful yeast genetic techniques, and complements existing reconstituted mammalian translation systems (Benne and Hershey, 1978; Pestova and Hetten, 2000; Pestova et al., 1998; Trachset et al., 1977). The following describes methods for synthesizing and purifying the components of the yeast initiation system and assays useful for its characterization.

引用

页码：111 / 145

页数：35

共 27 条

[1] Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining
Acker, MG
Shin, BS
Dever, TE
Lorsch, JR
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (13) : 8469 - 8475
[2] Pi release from elF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation
Algire, MA
Maag, D
Lorsch, JR
[J]. MOLECULAR CELL, 2005, 20 (02) : 251 - 262
[3] Development and characterization of a reconstituted yeast translation initiation system
Algire, MA
Maag, D
Savio, P
Acker, MG
Tarun, SZ
Sachs, AB
Asano, K
Nielsen, KH
Olsen, DS
Phan, L
Hinnebusch, AG
Lorsch, JR
[J]. RNA, 2002, 8 (03) : 382 - 397
[4] BENNE R, 1978, J BIOL CHEM, V253, P3078
[5] Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2
Choi, SK
Olsen, DS
Roll-Mecak, A
Martung, A
Remo, KL
Burley, SK
Hinnebusch, AG
Dever, TE
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (19) : 7183 - 7191
[6] Ligand interactions with eukaryotic translation initiation factor 2: Role of the gamma-subunit
Erickson, FL
Hannig, EM
[J]. EMBO JOURNAL, 1996, 15 (22) : 6311 - 6320
[7] Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase:: production and activity
Fechter, P
Rudinger, J
Giegé, R
Théobald-Dietrich, A
[J]. FEBS LETTERS, 1998, 436 (01) : 99 - 103
[8] Goode BL, 2002, METHOD ENZYMOL, V351, P433
[9] Initiator tRNA binding by e/aIF5B, the eukaryotic/archaeal homologue of bacterial initiation factor IF2
Guillon, L
Schmitt, E
Blanquet, S
Mechulam, Y
[J]. BIOCHEMISTRY, 2005, 44 (47) : 15594 - 15601
[10] Eukaryotic translation initiation factor 3 (eIF3) and eIF2 can promote mRNA binding to 40S subunits independently of eIF4G in yeast
Jivotovskaya, AV
Valásek, L
Hinnebusch, AG
Nielsen, KH
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (04) : 1355 - 1372

← 1 2 3 →