Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

被引：7

作者：

Yin, H

Chan, C

Garforth, J

Douglas, KT

Bolgar, MS

Gaskell, SJ

Fairlamb, AH

机构：

[1] UNIV MANCHESTER,DEPT PHARM,MANCHESTER M13 9PL,LANCS,ENGLAND

[2] UMIST,MICHAEL BARBER CTR MASS SPECTROMETRY,MANCHESTER M60 1QD,LANCS,ENGLAND

[3] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,LONDON WC1E 7HT,ENGLAND

来源：

CHEMICAL COMMUNICATIONS | 1996年 / 08期

关键词：

D O I：

10.1039/cc9960000973

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

引用

收藏

页码：973 / 974

页数：2

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