Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus

被引：80

作者：

Britton, P

Green, P

Kottier, S

Mawditt, KL

Penzes, Z

Cavanagh, D

Skinner, MA

机构：

[1] Division of Molecular Biology, Institute for Animal Health, Compton Laboratory, Compton, Newbury

[2] Centro Nacional de Biotecnologia, Consejo Sup. de Invest. Cientificas, Campus Universidad Autónoma, Cantoblanco

来源：

JOURNAL OF GENERAL VIROLOGY | 1996年 / 77卷

关键词：

D O I：

10.1099/0022-1317-77-5-963

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The bacteriophage T7 RNA polymerase gene was integrated into the fowlpox virus genome under the control of the vaccinia virus early/late promoter, P-7.5. The recombinant fowlpox virus, fpEFLT7pol, stably T7 RNA polymerase in avian and mammalian cells, allowing transient expression of transfected genes under the control of the T7 promoter. The recombinant fowlpox virus expressing T7 RNA polymerase offers an alternative to the widely used vaccinia virus vTF7-3, or the recently developed modified vaccinia virus Ankara (MVA) T7 RNA polymerase recombinant, a highly attenuated strain with restricted host-range. Recombinant fowlpox viruses have the advantage that as no infectious virus are produced from mammalian cells they do not have to be used under stringent microbiological safety conditions.

引用

收藏

页码：963 / 967

页数：5

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