High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance

被引：419

作者：

Lu, R ^{[1
]}

Malcuit, I ^{[1
]}

Moffett, P ^{[1
]}

Ruiz, MT ^{[1
]}

Peart, J ^{[1
]}

Wu, AJ ^{[1
]}

Rathjen, JP ^{[1
]}

Bendahmane, A ^{[1
]}

Day, L ^{[1
]}

Baulcombe, DC ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

来源：

EMBO JOURNAL | 2003年 / 22卷 / 21期

关键词：

gene silencing; HSP90; plant disease; resistance;

D O I：

10.1093/emboj/cdg546

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Virus-induced gene silencing was used to assess the function of random Nicotiana benthamiana cDNAs in disease resistance. Out of 4992 cDNAs tested from a normalized library, there were 79 that suppressed a hypersensitive response (HR) associated with Pto-mediated resistance against Pseudomonas syringae. However, only six of these clones blocked the Pto-mediated suppression of P.syringae growth. The three clones giving the strongest loss of Pto resistance had inserts corresponding to HSP90 and also caused loss of Rx-mediated resistance against potato virus X and N-mediated tobacco mosaic virus resistance. The role of HSP90 as a cofactor of disease resistance is associated with stabilization of Rx protein levels and could be accounted for in part by SGT1 and other cofactors of disease resistance acting as co-chaperones. This approach illustrates the potential benefits and limitations of RNA silencing in forward screens of gene function in plants.

引用

页码：5690 / 5699

页数：10

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