Snake Venomics of Bothriechis nigroviridis Reveals Extreme Variability among Palm Pitviper Venoms: Different Evolutionary Solutions for the Same Trophic Purpose

被引：53

作者：

Fernandez, Julian ^{[2
]}

Lomonte, Bruno ^{[2
]}

Sanz, Libia ^{[1
]}

Angulo, Yamileth ^{[2
]}

Maria Gutierrez, Jose ^{[2
]}

Calvete, Juan J. ^{[1
]}

机构：

[1] CSIC, Inst Biomed Valencia, Valencia 46010, Spain

[2] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose, Costa Rica

来源：

JOURNAL OF PROTEOME RESEARCH | 2010年 / 9卷 / 08期

关键词：

Bothriechis nigroviridis; black-speckled palm pitviper; snake venom proteomics; venomics; N-terminal sequencing; mass spectrometry; MOJAVE TOXIN; CROTALUS; BOTHROPS; RATTLESNAKES; ANTIVENOMICS; TOXICITY; DIET; NEUTRALIZATION; DIVERSITY; PEPTIDE;

D O I：

10.1021/pr100545d

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We report the proteomic characterization and biological activities of the venom of the black-speckled palm pitviper, Bothriechis nigroviridis, a neotropical arboreal pitviper from Costa Rica. In marked contrast to other Bothriechis species investigated, the venom of B. nigroviridis does not possess detectable Zn2+-dependent metalloproteinases, and is uniquely characterized by a high content of crotoxin-like PLA(2) and vasoactive peptides. These data suggest that different evolutionary solutions have evolved within the arboreal genus Bothriechis for the same trophic purpose. The venom from B. nigroviridis is devoid of hemorrhagic activity, has low edematogenic and coagulant effects, presents modest myotoxic and phospholipase A(2) activities, but has higher lethality than the venoms of other Bothriechis species. Neutralization of its lethal activity by an anti-Crotalus durissus terrificus antivenom confirmed the major role of crotoxin-like PLA(2) in B. nigroviridis venom-induced lethality.

引用

页码：4234 / 4241

页数：8

共 65 条

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