Non-radioisotopic and semi-quantitative procedure for terminal repeat amplification protocol

被引：51

作者：

Ohyashiki, JH ^{[1
]}

Ohyashiki, K ^{[1
]}

Sano, T ^{[1
]}

Toyama, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC MED,DEPT NEUROSURG,BUNKYO KU,TOKYO 113,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1996年 / 87卷 / 04期

关键词：

telomerase activity; fluorescence labeling;

D O I：

10.1111/j.1349-7006.1996.tb00225.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have used fluorescence-labeled primers and an auto-sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi-quantitative results are possible without using a photo-capture system, and 3) no radioisotope is needed.

引用

页码：329 / 331

页数：3

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