Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in Neurospora crassa using bimolecular fluorescence complementation

被引：27

作者：

Bardiya, Nirmala ^{[1
]}

Alexander, William G. ^{[1
]}

Perdue, Tony D. ^{[2
]}

Barry, Edward G. ^{[2
]}

Metzenberg, Robert L. ^{[3
]}

Pukkila, Patricia J. ^{[2
]}

Shiu, Patrick K. T. ^{[1
]}

机构：

[1] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA

[2] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

[3] Calif State Univ Northridge, Dept Biol, Northridge, CA 91330 USA

来源：

GENETICS | 2008年 / 178卷 / 01期

关键词：

D O I：

10.1534/genetics.107.079384

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Bimolecular fluorescence complementation (BiFC) is based on the complementation between two non-fluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa rising two genes involved in meiotic silencing by impaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.

引用

页码：593 / 596

页数：4

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