In Vitro Osteogenic Potential of an Experimental Calcium Silicate-based Root Canal Sealer

被引:43
作者
Bryan, Thomas E. [1 ]
Khechen, Khaled [2 ]
Brackett, Martha G. [3 ]
Messer, Regina L. W. [4 ]
El-Awady, Ahmed [4 ]
Primus, Carolyn M. [5 ]
Gutmann, James L. [6 ]
Tay, Franklin R. [1 ,3 ]
机构
[1] Med Coll Georgia, Sch Dent, Dept Endodont, Augusta, GA 30912 USA
[2] Dentsply Tulsa Dent Specialties, Tulsa, OK USA
[3] Med Coll Georgia, Sch Dent, Dept Oral Rehabil, Augusta, GA 30912 USA
[4] Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 USA
[5] Primus Consulting, Bradenton, FL USA
[6] Texas A&M Univ Syst, Hlth Sci Ctr, Baylor Coll Dent, Dept Endodont, Dallas, TX USA
关键词
Alkaline phosphatase; calcium silicate; cell culture; cytotoxicity; osteogenic potential; preosteoblasts; root canal sealer; MINERAL TRIOXIDE AGGREGATE; ENDODONTIC SEALERS; CYTOTOXICITY; CELLS; EXPRESSION; RESIN; BIOCOMPATIBILITY; CEMENT; VIVO; DIFFERENTIATION;
D O I
10.1016/j.joen.2010.03.034
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Objective: This in vitro study compared the cytotoxicity and osteogenic potential of an experimental calcium silicate based sealer with an epoxy resin based sealer (AH Plus; Dentsply Caulk, Milford, DE) and a zinc oxide eugenol based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Methods: Disks prepared from the respective sealer and from Teflon (negative control) were placed in direct contact with a MC3T3-E1 osteogenic cell line at 6 weekly intervals after immersion in a culture medium. Succinic dehydrogenase activities were evaluated using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Extracts from these sealers after the 6-week immersion period were investigated also by MTT assay. Aged sealers were then switched to an osteogenic medium for examination of the alkaline phosphatase activity and mineralization of extracellular matrices produced by the differentiated cells. Results: All sealers exhibited severe toxicity after 24 hours, after which toxicity decreased gradually over the experimental period except for Pulp Canal Sealer, which remained severely toxic. Toxicity of the extracts derived from the sealers was concentration dependent, with those derived from the experimental sealer being the least cytotoxic at a 1:10 dilution. Minimal alkaline phosphatase activity and no bone formation were seen with Pulp Canal Sealer. The production of alkaline phosphatase was less intense for the experimental sealer at 7 days. However, both AH Plus and the experimental sealer did not inhibit mineralization of the extracellular matrix after 28 days. Conclusion: The experimental calcium silicate based sealer may be regarded as minimally tissue irritating and does not interfere with bone regeneration even when it is inadvertently extruded through the apical constriction. (J Endod 2010;36:1163-1169)
引用
收藏
页码:1163 / 1169
页数:7
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