Nanogold-actuated biomimetic peroxidase for sensitized electrochemical immunoassay of carcinoembryonic antigen in human serum

被引：32

作者：

Chen, Huafeng ^{[1
,2
]}

Tang, Juan ^{[1
,2
]}

Su, Biting ^{[1
,2
]}

Chen, Guonan ^{[1
,2
]}

Huang, Jianxin ^{[3
,4
]}

Tang, Dianping ^{[1
,2
]}

机构：

[1] Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350108, Peoples R China

[2] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China

[3] Fujian Prov Hosp, Clin Lab, Fuzhou 350001, Fujian, Peoples R China

[4] Fujian Prov Hosp, Med Diagnost Lab, Fuzhou 350001, Fujian, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2010年 / 678卷 / 02期

关键词：

Biomimetic peroxidase; Carcinoembryonic antigen; Electrochemical immunosensor; Fe3O4; nanoparticles; Nanogold particles; Prussian blue; CARBON-PASTE INTERFACE; IMMUNOSENSOR ARRAY; POTENTIOMETRIC IMMUNOSENSOR; HORSERADISH-PEROXIDASE; CLINICAL IMMUNOASSAY; NANOPARTICLES; GOLD; ELECTRODE; SENSORS; BIOANALYSIS;

D O I：

10.1016/j.aca.2010.09.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A new electrochemical immunoassay protocol for the determination of carcinoembryonic antigen (CEA) in human serum was developed by means of immobilizing horseradish peroxidase-labeled anti-CEA antibodies (HRP-anti-CEA) on the nanogold/nano-Fe3O4 particles-functionalized polyion complex (PIC) membrane. With a one-step immunoassay format, the formed immunocomplex inhibited partly the bioactive center of the immobilized HRP, and decreased the HRP toward the reduction of H2O2. Nanogold-actuated nano-Fe3O4 mimetic peroxidase was used for the sensitivity improvement of the electrochemical immunosensor with Prussian blue (PB) as mediators. Under optimal conditions, the electrochemical immunosensor displayed a wide dynamic range of 0 1-220 ng mL(-1) with a relative low detection limit (LOD) of 10 pg mL(-1) CEA (at 3 sigma) Intra- and inter-assay show good precisions with a coefficient of variation (CV) lower than 7.8% and 7 3%, respectively No significant difference at the 95% confidence level was encountered in the analysis of 17 human serum specimens between the electrochemical immunosensor and automatic electrochemiluminescent (ECL) analyzer for the determination of CEA (C) 2010 Elsevier B V All rights reserved.

引用

页码：169 / 175

页数：7

共 55 条

[1]

ADDAYE F, 2008, SCIENCE, V321, P1795

[2]

Nano- and micro-structured assemblies for encapsulation of food ingredients [J].