Evaluation of an enzyme-linked immunosorbent assay based on binding inhibition for type-specific quantification of poliovirus neutralization-relevant antibodies

被引：14

作者：

Hashido, M

Horie, H

Abe, S

Doi, Y

Hashizume, S

Agboatwalla, M

Isomura, S

Nishio, O

Hagiwara, A

Inouye, S

机构：

[1] Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Shinjuku Ku, Tokyo 1628640, Japan

[2] Japan Poliomyelitis Res Inst, Tokyo 1890003, Japan

[3] Civil Hosp, Dept Pediat, Karachi, Pakistan

[4] Nagoya Univ, Sch Med, Dept Int Hlth, Nagoya, Aichi 4668560, Japan

[5] Natl Inst Publ Hlth, Dept Microbiol, Minato Ku, Tokyo 1080071, Japan

[6] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1999年 / 43卷 / 01期

关键词：

poliovirus; antibody; NT; ELISA;

D O I：

10.1111/j.1348-0421.1999.tb02375.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To detect neutralization-relevant antibodies against 3 types of poliovirus (PV) without using tissue cultures and live viruses, an enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody-binding inhibition was evaluated using sera from 80 vaccinated Japanese children and 60 Pakistani poliomyelitis patients, Compared with the neutralization test, the sensitivity of the inhibition ELISA was 100% (111/111) for detection of anti-PV1 antibody, 98.3% (118/120) for anti-PV2, and 96.5% (82/85) for anti-PV3, and the specificity was 93.1% (27/29), 100% (20/20), and 92.7% (51/55), respectively. Thus, the inhibition ELISA showed excellent potential as a seroepidemiologic tool in both vaccinated and naturally-infected populations.

引用

页码：73 / 77

页数：5

共 16 条

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