Rapid quantitation of potato glycoalkaloids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：34

作者：

Abell, DC ^{[1
]}

Sporns, P ^{[1
]}

机构：

[1] UNIV ALBERTA, DEPT AGR FOOD & NUTR SCI, EDMONTON, AB T6G 2P5, CANADA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1996年 / 44卷 / 08期

关键词：

MALDI; chaconine; solanine; tomatine;

D O I：

10.1021/jf9507102

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The potential for application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to the quantification of potato-glycoalkaloids was investigated. A MALDI-TOF MS method was developed for the analysis of alpha-chaconine and alpha-solanine using tomatine as an internal standard. Quantitative analysis of a number of potato cultivars by MALDI-TOF MS and high-performance liquid chromatography (HPLC) showed high correlation (R(2) = 0.98) between the two methods. The MALDI-TOF MS method developed provides a very rapid analysis, requires very little sample preparation, and is suitable for routine glycoalkaloid analysis.

引用

页码：2292 / 2296

页数：5

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