Genes of Bacillus cereus and Bacillus anthracis encoding proteins of the exosporium

被引:145
作者
Todd, SJ [1 ]
Moir, AJG [1 ]
Johnson, MJ [1 ]
Moir, A [1 ]
机构
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
关键词
D O I
10.1128/JB.185.11.3373-3378.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The exosporium is the outermost layer of spores of Bacillus cereus and its close relatives Bacillus anthracis and Bacillus thuringiensis. For these pathogens, it represents the surface layer that makes initial contact with the host. To date, only the BcIA glycoprotein has been described as a component of the exosporium; this paper defines 10 more tightly associated proteins from the exosporium of B. cereus ATCC 10876, identified by N-terminal sequencing of proteins from purified, washed exosporium. Likely coding sequences were identified from the incomplete genome sequence of B. anthracis or B. cereus ATCC 14579, and the precise corresponding sequence from B. cereus ATCC 10876 was defined by PCR and sequencing. Eight genes encode likely structural components (exsB, exsC, exsD, exsE, exsF, exsG, exsJ, and cotE). Several proteins of the exosporium are related to morphogenetic and outer spore coat proteins of B. subtilis, but most do not have homologues in B. subtilis. ExsE is processed from a larger precursor, and the CotE homologue appears to have been C-terminally truncated. ExsJ contains a domain of GXX collagen-like repeats, like the BcIA exosporium protein of B. anthracis. Although most of the exosporium genes are scattered on the genome, bclA and exsF are clustered in a region flanking the rhamnose biosynthesis operon; rhamnose is part of the sugar moiety of spore glycoproteins. Two enzymes, alanine racemase and nucleoside hydrolase, are tightly adsorbed to the exosporium layer; they could metabolize small molecule germinants and may reduce the sensitivity of spores to these, limiting premature germination.
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页码:3373 / 3378
页数:6
相关论文
共 24 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Germination of Bacillus cereus spores in response to L-alanine and to inosine:: the roles of gerL and gerQ operons [J].
Barlass, PJ ;
Houston, CW ;
Clements, MO ;
Moir, A .
MICROBIOLOGY-SGM, 2002, 148 :2089-2095
[3]   PARACRYSTALLINE SHEETS REAGGREGATED FROM SOLUBILIZED EXOSPORIUM OF BACILLUS-CEREUS [J].
BEAMAN, TC ;
PANKRATZ, HS ;
GERHARDT, P .
JOURNAL OF BACTERIOLOGY, 1971, 107 (01) :320-+
[4]   The measurement of Bacillus mycoides spore adhesion using atomic force microscopy, simple counting methods, and a spinning disk technique [J].
Bowen, WR ;
Fenton, AS ;
Lovitt, RW ;
Wright, CJ .
BIOTECHNOLOGY AND BIOENGINEERING, 2002, 79 (02) :170-179
[5]   Characterization of the exosporium of Bacillus cereus [J].
Charlton, S ;
Moir, AJG ;
Baillie, L ;
Moir, A .
JOURNAL OF APPLIED MICROBIOLOGY, 1999, 87 (02) :241-245
[6]   Role of the gerI operon of Bacillus cereus 569 in the response of spores to germinants [J].
Clements, MO ;
Moir, A .
JOURNAL OF BACTERIOLOGY, 1998, 180 (24) :6729-6735
[7]  
DESROSIER JP, 1984, J GEN MICROBIOL, V130, P935
[8]   The InhA2 metalloprotease of Bacillus thuringiensis strain 407 is required for pathogenicity in insects infected via the oral route [J].
Fedhila, S ;
Nel, P ;
Lereclus, D .
JOURNAL OF BACTERIOLOGY, 2002, 184 (12) :3296-3304
[9]   DETERMINATION OF CARBOHYDRATE PROFILES OF BACILLUS-ANTHRACIS AND BACILLUS-CEREUS INCLUDING IDENTIFICATION OF O-METHYL METHYLPENTOSES BY USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].
FOX, A ;
BLACK, GE ;
FOX, K ;
ROSTOVTSEVA, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (04) :887-894
[10]   A GLYCOPROTEIN MULTIMER FROM BACILLUS-THURINGIENSIS SPORANGIA - DISSOCIATION INTO SUBUNITS AND SUGAR COMPOSITION [J].
GARCIAPATRONE, M ;
TANDECARZ, JS .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 1995, 145 (01) :29-37