The membrane domain of complex I is not assembled in the stopper mutant E35 of Neurospora

被引：12

作者：

Alves, PC ^{[1
]}

Videira, A

机构：

[1] Univ Porto, Inst Ciencias Biomed Abel Salazar, Inst Biol Mol & Celular, P-4100 Porto, Portugal

[2] Univ Porto, Unidade Multidisciplinar Invest Biomed, P-4100 Porto, Portugal

来源：

BIOCHEMISTRY AND CELL BIOLOGY | 1998年 / 76卷 / 01期

关键词：

mitochondria; complex I; stopper mutants; Neurospora crassa;

D O I：

10.1139/bcb-76-1-139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The assembly of mitochondrial NADH : ubiquinone oxidoreductase (complex I) was studied in the E35 stopper mutant of Neurospora crassa at different times during growth in liquid media. Assembly of complex I as well as of its membrane domain is impaired in this strain throughout the growth period. Nevertheless, a structure that resembles the peripheral arm of the enzyme is still formed in the mitochondria of this mutant. The absence of the membrane domain of complex I in E35 can be attributed to the specific deletion of the mitochondrial ND2 and ND3 subunits of the enzyme.

引用

页码：139 / 143

页数：5

共 44 条

[1] DUPLICATION OF THE TRANSFER RNAMMET AND TRANSFER RNACYS GENES AND OF FRAGMENTS OF A GENE ENCODING A SUBUNIT OF THE NADH DEHYDROGENASE COMPLEX IN NEUROSPORA-GRASSA MITOCHONDRIAL-DNA [J].

AGSTERIBBE, E ;

HARTOG, M ;

DEVRIES, H .

CURRENT GENETICS, 1989, 15 (01) :57-62

[2] CHARACTERIZATION OF A NOVEL PLASMID-LIKE ELEMENT IN NEUROSPORA-CRASSA DERIVED MOSTLY FROM THE MITOCHONDRIAL-DNA [J].

ALMASAN, A ;

MISHRA, NC .

NUCLEIC ACIDS RESEARCH, 1990, 18 (19) :5871-5877

[3]

ALMASAN A, 1988, GENETICS, V120, P935

[4]

ALVES PC, 1994, J BIOL CHEM, V269, P7777

[5] DELETION MUTANTS OF NEUROSPORA-CRASSA MITOCHONDRIAL-DNA AND THEIR RELATIONSHIP TO THE STOP-START GROWTH PHENOTYPE [J].

BERTRAND, H ;

COLLINS, RA ;

STOHL, LL ;

GOEWERT, RR ;

LAMBOWITZ, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (10) :6032-6036

[6]

BERTRAND H, 1969, GENETICS, V61, P643

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8]

BROWN MD, 1992, GENETICS, V130, P163

[9]

daSilva MV, 1996, MOL GEN GENET, V252, P177

[10]

Davis R. H., 1970, METHODS ENZYMOLOGY A, V17, P79, DOI [DOI 10.1016/0076-6879(71)17168-6, 10.1016/0076-6879(71)17168-6]

← 1 2 3 4 5 →