DNA-templated nanotube localization

Carbon nanotubes are nanometer-scale materials with important properties, but their use in nanofabrication will require further development of methods for controlled positioning at well-defined locations on surfaces. We have devised an approach for specifically localizing single-walled carbon nanotubes (SWNTs) onto 1-pyrenemethylamine (PMA)-decorated λ-DNA molecules aligned on Si surfaces. PMA is used as a bridging compound because its amine group is attracted electrostatically to the negatively charged phosphate backbone of DNA, while the pyrenyl group in PMA interacts with SWNT surfaces through π-stacking forces. From a total of 60 atomic force microscopy images obtained on three different substrates, we determined that 63% of SWNTs observed on the surfaces were anchored along DNA, and these nanotubes covered ∼5% of the total DNA length. DNA-templated nanopositioning offers intriguing possibilities for the bottom-up assembly of materials at the nanometer scale. Copyright © 2003 American Chemical Society.