Circularization of mRNA by eukaryotic translation initiation factors

被引：752

作者：

Wells, SE

Hillner, PE

Vale, RD

Sachs, AB

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Calif San Francisco, Dept Pharmacol, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Pharmacol, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Dept Biochem, San Francisco, CA 94143 USA

来源：

MOLECULAR CELL | 1998年 / 2卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S1097-2765(00)80122-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, elF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor elF4G. Here, we demonstrate the reconstitution of an elF4E/elF4G/Pab1p complex with recombinant proteins, and show by atomic force microscopy that the complex can circularize capped, polyadenylated RNA. Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell.

引用

收藏

页码：135 / 140

页数：6

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