Effects of estrous cyclicity on the expression of the galanin receptor Gal-R1 in the rat preoptic area:: A comparison with the male

被引：32

作者：

Faure-Virelizier, C ^{[1
]}

Croix, D ^{[1
]}

Bouret, S ^{[1
]}

Prévot, V ^{[1
]}

Reig, S ^{[1
]}

Beauvillain, JC ^{[1
]}

Mitchell, V ^{[1
]}

机构：

[1] INSERM U422, F-59045 Lille, France

来源：

ENDOCRINOLOGY | 1998年 / 139卷 / 10期

关键词：

D O I：

10.1210/en.139.10.4127

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Variations in the number of galanin receptor (Gal-R1)-expressing cells and levels of Gal-R1 messenger RNA (mRNA) were determined in the preoptic area in intact female rats throughout the phases of the estrous cycle and compared with those in the male. Female and male Wistar rats were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were hybridized with a S-35-labeled antisense Gal-R1 riboprobe. The number of Gal-R1 mRNA-expressing cells was lower in the rostral preoptic area than in the medial preoptic area. During the estrous cycle, the highest number of Gal-R1 mRNA-expressing cells in the rostral preoptic region was detected at 0800 h on proestrus, whereas in the medial preoptic area, the maximum number was observed at 1800 h on estrus. Gal-R1 mRNA levels in individual cells mere low during diestrus and increased at estrus in both areas. In the male, the number of mRNA-expressing cells and the hybridization signal were significantly lower than those in females during estrus. The results demonstrate that Gal-R1 gene expression in the preoptic area varies during the estrous cycle and is low in males. Short term treatment of ovariectomized rats with estradiol plus progesterone caused significantly decreased preoptic Gal-R1 mRNA levels compared with those after treatment with estrogen only. These observations suggest that in the preoptic area, expression of Gal-R1 is influenced by progesterone. The variation in Gal-R1 expression is likely to influence the extent to which galanin can influence the preoptic cells implicated in the control of neighboring GnRH cells.

引用

页码：4127 / 4139

页数：13

共 38 条

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