Antileishmanial activity of an indole alkaloid from Peschiera australis

被引：142

作者：

Delorenzi, JC

Attias, M

Gattass, CR

Andrade, M

Rezende, C

Pinto, AD

Henriques, AT

Bou-Habib, DC

Saraiva, EMB

机构：

[1] Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Mayer, Rio De Janeiro, Brazil

[2] Inst Microbiol, Dept Imunol, Lab Imunobiol Leishmanioses, Rio De Janeiro, Brazil

[3] Fed Univ Rio De Janeiro, Inst Quim, Dept Quim Organ, Rio De Janeiro, Brazil

[4] Univ Fed Rio Janeiro, Inst Quim, Dept Quim Organ, Porto Alegre, RS, Brazil

[5] Univ Fed Rio Grande Sul, Fac Farm, Porto Alegre, RS, Brazil

[6] Fdn Oswaldo Cruz, Ctr Pesquisas Goncalo Moniz, Salvador, BA, Brazil

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 05期

关键词：

D O I：

10.1128/AAC.45.5.1349-1354.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we show the leishmanicidal effects of a chloroform fraction (CLF) and a purified indole alkaloid obtained from crude stem extract of Peschiera australis against Leishmania amazonensis, a causative agent of cutaneous leishmaniasis in the New World. In a bioassay-guided chemical fractionation, the leishmanicidal activity in CLF completely and irreversibly inhibited promastigote growth. This fraction was also active against amastigotes in infected murine macrophages, Chemical analysis of CLP identified an iboga-type indole alkaloid coronaridine as one of its major compounds. Coronaridine showed potent antileishmanial activity, inhibiting promastigote and amastigote growth. Promastigotes and amastigotes treated with CLF or coronaridine showed pronounced alterations in their mitochondria as assessed by transmission electron microscopy.

引用

页码：1349 / 1354

页数：6

共 28 条

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