Cell-culture compatible silk fibroin scaffolds concomitantly patterned by freezing conditions and salt concentration

被引：20

作者：

Byette, Frederic ^{[2
]}

Bouchard, Frederic ^{[2
]}

Pellerin, Christian ^{[3
,4
]}

Paquin, Joanne ^{[2
]}

Marcotte, Isabelle ^{[1
,2
]}

Mateescu, Mircea A. ^{[1
,2
]}

机构：

[1] Univ Quebec, Dept Chem, Downtown Stn, Montreal, PQ H3C 3P8, Canada

[2] Univ Quebec, Dept Chim Pharmaqam NanoQAM, Montreal, PQ H3C 3P8, Canada

[3] Univ Montreal, Dept Chim, Montreal, PQ H3C 3J7, Canada

[4] Ctr Self Assembled Chem Struct, Montreal, PQ H3C 3J7, Canada

来源：

POLYMER BULLETIN | 2011年 / 67卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Silk fibroin; Scaffold; Cell culture; Freeze-drying; Crystal nucleation and growth; Sodium chloride concentration; Freezing temperature; Mechanical properties; Spectroscopic characterization; MESENCHYMAL STEM-CELLS; BOMBYX-MORI; POROUS STRUCTURES; PHASE-SEPARATION; SOLID-STATE; BIOMATERIALS; FABRICATION; SPECTROSCOPY; WATER; FIBROBLASTS;

D O I：

10.1007/s00289-010-0438-z

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The morphology of freeze-dried silk fibroin 3D-scaffolds was modified by varying both the NaCl concentration and the freezing temperature of the silk fibroin solution prior to lyophilization. Scanning electron micrographs showed that slow freezing at -22 A degrees C generated sponge-like interconnected porous networks, whereas fast freezing at -73 A degrees C formed stacked leaflet structures. The presence of millimolar NaCl (50-250 mM) increased the porosity of the scaffolds and generated small outgrowths at their surface, depending on the freezing regime. Our results suggest that the morphological differences seen between the materials likely depend on ice and NaCl hydrate crystal nucleation and growth mechanisms. Infrared spectroscopy and X-ray diffraction analyses revealed that the salt concentration and freezing conditions induced no structural changes in fibroin. The seeding of P19 embryonic carcinoma cells showed that the presence of salt and freezing conditions influenced the cell distribution into the scaffolds, with salt addition increasing the access of cells to deeper regions.

引用

页码：159 / 175

页数：17

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