Large-scale production in Pichia pastoris of the recombinant vaccine Gavac(TM) against cattle tick

被引：147

作者：

Canales, M ^{[1
]}

Enriquez, A ^{[1
]}

Ramos, E ^{[1
]}

Cabrera, D ^{[1
]}

Dandie, H ^{[1
]}

Soto, A ^{[1
]}

Falcon, V ^{[1
]}

Rodriguez, M ^{[1
]}

delaFuente, J ^{[1
]}

机构：

[1] INT CTR GENET ENGN & BIOTECHNOL, ANALYT UNIT, HAVANA 6, CUBA

来源：

VACCINE | 1997年 / 15卷 / 04期

关键词：

Boophilus microplus; vaccine; rBm86; protein; Gavac;

D O I：

10.1016/S0264-410X(96)00192-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A gene coding for the Bm86 tick protein was recently cloned, expressed in Pichia pastoris and shown to induce an immunological response in cattle against ticks. Moreover, the Gavac(TM) vaccine (Heber Biotec S.A., Havana, Cuba), which contains this recombinant protein, has proved to control the Boophilus microplus populations under field conditions. This paper reviews the development and large-scale production of this vaccine, the efficacy of the resulting product and the strategy followed in designing its production plant. The production plant fulfills biosafety requirements and GMP. (C) 1997 Elsevier Science Ltd.

引用

页码：414 / 422

页数：9

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