The aminoacceptor stem of the yeast tRNALys contains determinants of mitochondrial import selectivity

被引：24

作者：

Kazakova, HA

Entelis, NS

Martin, RP

Tarassov, IA

机构：

[1] CNRS, UPR 9005, Div Cellulaire & Dev, F-67084 Strasbourg, France

[2] Moscow MV Lomonosov State Univ, Fac Biol, Div Mol Biol, Moscow 119899, Russia

来源：

FEBS LETTERS | 1999年 / 442卷 / 2-3期

基金：

俄罗斯基础研究基金会;

关键词：

tRNA; mitochondrial import; aminoacceptor stem; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(98)01653-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast tRNA(CUU)(Lys) is nucleus-encoded and is partially imported into the mitochondria, Another lysine isoacceptor, tRNA(SUU)(Lys), is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases, We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of mutant transcripts and by expression of mutant tRNA genes in vivo we show here that the first base pair (1:72) and the discriminator base 73 are also relevant to import selectivity. Replacement of bases 1:72 in tRNA(SUU)(Lys) by those of tRNA(CUU)(Lys) makes it importable with a transport efficiency similar to natural. (C) 1999 Federation of European Biochemical Societies.

引用

页码：193 / 197

页数：5

共 19 条

[1] Role of an RNA-binding protein in import of tRNA into Leishmania mitochondria [J].