Evaluation of an intracellular-activated promoter for the generation of live Salmonella recombinant vaccines

被引：26

作者：

Husseiny, MI ^{[1
]}

Hensel, M ^{[1
]}

机构：

[1] FAU Erlangen Nurnberg, Inst Klin Mikrobiol Immunol & Hyg, D-91054 Erlangen, Germany

来源：

VACCINE | 2005年 / 23卷 / 20期

关键词：

Salmonella; live recombinant vaccine; genetic engineering; heterologous antigen expression;

D O I：

10.1016/j.vaccine.2004.11.035

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Salmonella enterica serovar Typhimurium is a versatile host organism for the generation of recombinant live vaccines for mucosal immunization. We investigated the performance of an intracellular-activated promoter for expression of heterologous antigens by Salmonella carrier strains. We observed that intracellular expressed antigens were more efficient in elicitation of humoral and cellular immune responses than constitutively expressed antigens. Furthermore, the intracellular-activated promoter was more efficient in combination with a carrier strain deficient in SPI2 gene sseC than with the conventional aroA strain. We propose that intracellular-activated promoters will be useful tools for construction of efficient recombinant live vaccines. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2580 / 2590

页数：11

共 26 条

[1] Regulated antigen expression in live recombinant Salmonella enterica serovar typhimurium strongly affects colonization capabilities and specific CD4+-T-cell responses [J].

Bumann, D .

INFECTION AND IMMUNITY, 2001, 69 (12) :7493-7500

[2] CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].

CHANG, ACY ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156

[3] Construction, characterization, and immunogenicity of an attenuated Salmonella enterica serovar typhimurium pgtE vaccine expressing fimbriae with integrated viral epitopes from the spiC promoter [J].

Chen, HQ ;

Schifferli, DM .

INFECTION AND IMMUNITY, 2003, 71 (08) :4664-4673

[4] Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival [J].

Cirillo, DM ;

Valdivia, RH ;

Monack, DM ;

Falkow, S .

MOLECULAR MICROBIOLOGY, 1998, 30 (01) :175-188

[5] Environmental regulation of Salmonella pathogenicity island 2 gene expression [J].

Deiwick, J ;

Nikolaus, T ;

Erdogan, S ;

Hensel, M .

MOLECULAR MICROBIOLOGY, 1999, 31 (06) :1759-1773

[6] Unravelling the biology of macrophage infection by gene expression profiling of intracellular Salmonella enterica [J].

Eriksson, S ;

Lucchini, S ;

Thompson, A ;

Rhen, M ;

Hinton, JCD .

MOLECULAR MICROBIOLOGY, 2003, 47 (01) :103-118

[7] Type III secretion of Salmonella enterica serovar Typhimurium translocated effectors and SseFG [J].

Hansen-Wester, I ;

Stecher, B ;

Hensel, M .

INFECTION AND IMMUNITY, 2002, 70 (03) :1403-1409

[8] THE MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED ANTIGEN RECEPTOR ON T-CELLS .1. ISOLATION WITH A MONOCLONAL-ANTIBODY [J].

HASKINS, K ;

KUBO, R ;

WHITE, J ;

PIGEON, M ;

KAPPLER, J ;

MARRACK, P .

JOURNAL OF EXPERIMENTAL MEDICINE, 1983, 157 (04) :1149-1169

[9] Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages [J].

Hensel, M ;

Shea, JE ;

Waterman, SR ;

Mundy, R ;

Nikolaus, T ;

Banks, G ;

Vazquez-Torres, A ;

Gleeson, C ;

Fang, FC ;

Holden, DW .

MOLECULAR MICROBIOLOGY, 1998, 30 (01) :163-174

[10] Superior efficacy of secreted over somatic antigen display in recombinant Salmonella vaccine induced protection against listeriosis [J].

Hess, J ;

Gentschev, I ;

Miko, D ;

Welzel, M ;

Ladel, C ;

Goebel, W ;

Kaufmann, SHE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (04) :1458-1463

← 1 2 3 →