Drosophila mitochondrial transcription factor A (d-TFAM) is dispensable for the transcription of mitochondrial DNA in Kc167 cells

被引：37

作者：

Goto, A ^{[1
]}

Matsushima, Y ^{[1
]}

Kadowaki, T ^{[1
]}

Kitagawa, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Grad Course Regulat Biol Signals, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

BIOCHEMICAL JOURNAL | 2001年 / 354卷

关键词：

HMG box; mitochondria; RNA interference;

D O I：

10.1042/0264-6021:3540243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned cDNA encoding Drosophila mitochondrial (mt) transcription factor A (d-TFAM). RNA interference (RNAi) of d-TFAM by lipofection of haemocyte-derived Kc167 cells with double-stranded RNA reduced d-TFAM to less than 5% of the normal level. Reflecting the ability of TFAM to stabilize mtDNA, RNAi of d-TFAM reduced mtDNA to 40%. Nonetheless, transcription of the ND2 and ND5 genes and their mRNAs remained unchanged for 8 days of the duration of RNAi. We thus show that d-TFAM is not essential for the transcription of Drosophila mtDNA.

引用

页码：243 / 248

页数：6

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