Epitope analysis of staphylococcal enterotoxin A using different synthetic peptides

被引：6

作者：

Hu, DL

Imai, A

Ono, K

Sasaki, S

Nakane, A

Sugii, S

Shinagawa, K

机构：

[1] Iwate Univ, Fac Agr, Dept Vet Med, Morioka, Iwate 0200066, Japan

[2] Hirosaki Univ, Sch Med, Dept Bacteriol, Hirosaki, Aomori 0368216, Japan

[3] Univ Osaka Prefecture, Coll Agr, Dept Vet Sci, Osaka 5998531, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1998年 / 60卷 / 09期

关键词：

enterotoxin; epitope; peptide; Staphylococcus aureus;

D O I：

10.1292/jvms.60.993

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The antigenic determinants (or epitopes) of staphylococcal enterotoxin A (SEA) were analyzed using synthetic peptides and rabbit antibodies to their corresponding peptides. Of 12 different synthetic peptides tested, peptides A-1 (corresponding to the amino acid sequence 1-20), A-5 (81-100), and A-8 (141-160) were reactive with anti-SEA antibodies. However, all synthetic peptides were found to elicit antibodies reactive with native SEA molecule. These findings suggest that native SEA molecule contains at least 3 different antigenic determinants.

引用

页码：993 / 996

页数：4

共 17 条

[1] PURIFICATION AND SOME PHYSICOCHEMICAL PROPERTIES OF ENTEROTOXIN C STAPHYLOCOCCUS AUREUS STRAIN 361 [J].