Effects of dietary supplementation with arachidonic acid rich oils on nerve conduction and blood flow in streptozotocin-diabetic rats

Diabetes mellitus is associated with defective essential fatty acid desaturation. In experimental models this contributes to characteristic reductions in peripheral nerve conduction velocity (NCV) and blood flow, which may be corrected by dietary supplementation with gamma-linolenic acid (GLA) rich oils to bypass the Delta-6 desaturation deficit. There is debate about the mechanism of this improvement, including whether it depends on synthesis of series 1 prostanoids derived from di-homo GLA or series 2 prostanoids from arachidonic acid (ARA). The aim was to assess the efficacy of two ARA-rich (similar to 39% content) oils in correcting neurovascular dysfunction in streptozotocin-induced diabetic rats. After 6 weeks of untreated diabetes, rats were treated for a further 2 weeks with 1% dietary oil supplements before assessment of sciatic motor NCV and endoneurial blood flow. NCV was 19% reduced in diabetic rats and this was largely (similar to 86%) corrected by both oil treatments. A 48% deficit in endoneurial nutritive blood flow with diabetes was similar to 70% reversed by the two oils, vascular conductance being in the non-diabetic range. Thus, nerve conduction and perfusion deficits in diabetic rats are corrected by ARA-rich oil treatment. The magnitudes of these changes were similar to expectations based on previous studies of GLA-rich oils, therefore it is likely that the neurovascular effect of increased synthesis of series 2 prostanoids makes a major contribution to the beneficial action of n-6 essential fatty acids in experimental diabetic neuropathy.