Expression of the major heat shock proteins DnaK and GroEL in Streptococcus pyogenes:: A comparison to Enterococcus faecalis and Staphylococcus aureus

被引:12
作者
Laport, MS [1 ]
de Castro, ACD [1 ]
Villardo, A [1 ]
Lemos, JAC [1 ]
Bastes, MDF [1 ]
Giambiagi-deMarval, M [1 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Dept Med Microbiol, CCS, BR-21491590 Rio De Janeiro, Brazil
关键词
D O I
10.1007/s002840110215
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
One of the outstanding problems in the field of heat shock response has been to elucidate the mechanism underlying the induction of heat shock proteins (HSPs). In this work, we initiate an analysis of the expression of heat shock groEL and dnaK genes and their promoters in S. pyogenes. The synthesis of total cellular proteins was studied upon transfer of a log-phase culture from 37 degreesC to 42 degreesC by performing 5-min pulse-labeling experiments with S-35-Met. The heat shock responses in the pathogenic Gram-positive cocci, Enterococcus faecalis and Staphylococcus aureus, were also analyzed.
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页码:264 / 268
页数:5
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