Variety of TEM-, SHV-, and CTX-M-type β-lactamases present in recent clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae from Taiwan

被引：38

作者：

Ma, L

Chang, FY

Fung, CP

Chen, TL

Lin, JC

Lu, PL

Huang, LY

Chang, JC

Siu, LK

机构：

[1] Natl Hlth Res Inst, Div Clin Res, Taipei 11529, Taiwan

[2] Tri Serv Gen Hosp, Natl Def Med Ctr, Div Infect Dis & Trop Med, Dept Internal Med, Taipei, Taiwan

[3] Taipei Vet Gen Hosp, Infect Dis Sect, Dept Med, Taipei, Taiwan

[4] Natl Yang Ming Univ, Taipei 112, Taiwan

[5] Kaohsiung Med Univ Hosp, Dept Internal Med, Taipei, Taiwan

来源：

MICROBIAL DRUG RESISTANCE | 2005年 / 11卷 / 01期

关键词：

D O I：

10.1089/mdr.2005.11.31

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

A total of 171 hospitals' isolates of E. coli, K. pneumoniae, and E. cloacae with a minimum inhibitory concentration (MIC) of >= 2 mu g/ml for ceftazidime or cefotaxime were evaluated for the production of beta-lactamases. PCR amplification with specific primers for the bla(SHV), bla(TEM), and bla(CTX) genes revealed that a total of 53, 81, and 43 of these genes were amplified, respectively. Sequencing results confirmed that TEM-1, CTX-M-3 and -14, SHV-1, -5, -11, -12, and -33, OXY-1a, and LEN-1 were presented among these isolates. No specific large cluster of isolates carried the same beta-lactamases, indicating the wide diversity of the collected strains. Plasmid spread between E. coli and K. neumoniae was identified in few isolates. Combinations of TEM, SHV, and CTX beta-lactamase genes, including extended-spectrum beta-lactamase genes, were observed in all three species.

引用

页码：31 / 39

页数：9

共 33 条

[1]

AUSUBEL FM, 1995, MINIPREP BACTERIAL G

[2]

Are SHV β-lactamases universal in Klebsiella pneumoniae? [J].