Allozyme and RAPD analysis of the genetic diversity and geographic variation in wild populations of the American chestnut (Fagaceae)

被引:58
作者
Huang, HW
Dane, F
Kubisiak, TL
机构
[1] Auburn Univ, Dept Hort, Auburn, AL 36849 USA
[2] Chinese Acad Sci, Wuhan Inst Bot, Moshan Wuhan 430074, Hubei, Peoples R China
[3] USDA, Forest Serv, So Res Stn, So Inst Forest Genet, Saucier, MS 39574 USA
关键词
allozyme variation; Castanea dentata; conservation; Fagaceae; geographic variation; genetic diversity; population structure; random amplified polymorphic DNA (RAPD);
D O I
10.2307/2446368
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Genetic variation among 12 populations of the American chestnut (Castanea dentata) was investigated. Population genetic parameters estimated from allozyme variation suggest that C. dentata at both the population and species level has narrow genetic diversity as compared to other species in the genus. Average expected heterozygosity was relatively low for the population collected in the Black Rock Mountain State Park, Georgia (H-e = 0.096 +/- 0.035), and high for the population in east central Alabama (H-e = 0.196 +/- 0.048). Partitioning of the genetic diversity based on 18 isozyme loci showed that similar to 10% of the allozyme diversity resided among populations. Cluster analysis using unweighted pair-group method using arithmetric averages of Rogers' genetic distance and principal components analysis based on allele frequencies of both isozyme and RAPD loci revealed four groups: the southernmost population, south-central Appalachian populations, northcentral Appalachian populations, and northern Appalachian populations. Based on results presented in this study, a conservation strategy and several recommendations related to the backcross breeding aimed at restoring C. dentata are discussed.
引用
收藏
页码:1013 / 1021
页数:9
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