Two distinct human POM121 genes: Requirement for the formation of nuclear pore complexes

被引：43

作者：

Funakoshi, Tomoko

Maeshima, Kazuhiro

Yahata, Kazuhide

Sugano, Sumio

Imamoto, Fumio

Imamoto, Naoko

机构：

[1] RIKEN, Discovery Res Inst, Cellular Dynam Lab, Wako, Saitama 3510198, Japan

[2] Osaka Univ, BIKEN, Dept Mol Biol, Osaka, Japan

[3] Univ Tokyo, Inst Med Sci, Tokyo, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 25期

关键词：

nuclear pore complex; Pom121; nuclear structure; RNAi; segmental-duplication;

D O I：

10.1016/j.febslet.2007.09.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike ro- dent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental-duplications in this region during human evolution. In HeLa cells, two "full-length" Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121-depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：4910 / 4916

页数：7

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