Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

被引：13

作者：

Couto, SR

Rättö, M

Domínguez, A

Sanromán, A

机构：

[1] Univ Vigo, Dept Chem Engn, E-36200 Vigo, Spain

[2] VTT, Biotechnol & Food Res, FIN-02044 Espoo, Finland

来源：

PROCESS BIOCHEMISTRY | 2001年 / 36卷 / 10期

关键词：

bioreactor; lignin peroxidase; manganese-dependent peroxidase; Phanerochaete chrysosporium; polypropylene sponges; solid-state fermentation;

D O I：

10.1016/S0032-9592(01)00139-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LIP) activities. Moreover, both MnP and Lip profiles were more regular in the modified bioreactor. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：995 / 999

页数：5

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