Unrip is a component of SMN complexes active in snRNP assembly

被引：66

作者：

Carissimi, C

Baccon, J

Straccia, M

Chiarella, P

Maiolica, A

Sawyer, A

Rappsilber, J

Pellizzoni, L ^{[1
]}

机构：

[1] CNR, Inst Cell Biol, Dulbecco Telethon Inst, I-00016 Rome, Italy

[2] Univ Penn, Sch Med, Philadelphia, PA 19104 USA

[3] EMBL, Monoclonal Antibody Core Facil, I-00016 Rome, Italy

[4] FIRC, Inst Mol Oncol Fdn, I-20139 Milan, Italy

来源：

FEBS LETTERS | 2005年 / 579卷 / 11期

关键词：

survival of motor neurons; spinal muscular atrophy; small nuclear ribonucleoprotein; unr-interacting protein;

D O I：

10.1016/j.febslet.2005.03.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting protein (unrip), a GH-WD protein of unknown function, as a component of the SMN complex that interacts directly with Gemin6 and Gemin7. Unrip also binds a subset of Sm proteins, and unrip-containing SMN complexes are necessary and sufficient to mediate the assembly of spliceosomal snRNPs. These results demonstrate that unrip functions in the pathway of snRNP biogenesis and is a marker of cellular SMN complexes active in snRNP assembly. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2348 / 2354

页数：7

共 28 条

[1] Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex [J].