Integration host factor positively regulates cysJIH transcription

被引：5

作者：

Sirko, A ^{[1
]}

Weglenska, A ^{[1
]}

Hulanicka, D ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

来源：

MOLECULAR AND GENERAL GENETICS | 1998年 / 258卷 / 1-2期

关键词：

integration host factor; ihfA; cysJIH; Escherichia coli K-12; Salmonella typhimurium;

D O I：

10.1007/s004380050720

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the identification of integration host factor (IHF) as an additional element involved in the regulation of the cysJIH promoter of Salmonella typhimurium and Escherichia coli. An IHF-binding site was located in the regulatory region of the cysJIH promoter by using two methods, protection against DNase I digestion and hydroxyl radical cleavage. The positive influence of IHF on in vitro run-off transcription from the cysJIH promoter is shown. The in vivo observations suggest that IHF is necessary for full expression of cysJIH in stationary phase but not during exponential growth.

引用

收藏

页码：174 / 177

页数：4

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