A recombinase-mediated transcriptional induction system in transgenic plants

被引:91
作者
Hoff, T [1 ]
Schnorr, KM [1 ]
Mundy, J [1 ]
机构
[1] Univ Copenhagen, Dept Plant Physiol, DK-1353 Copenhagen K, Denmark
关键词
Arabidopsis; Cre-lox; Cre-recombinase; heat shock; inducible; vector;
D O I
10.1023/A:1006402308365
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.
引用
收藏
页码:41 / 49
页数:9
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