The Pharmacological Effects of Morroniside and Loganin Isolated from Liuweidihuang Wan, on MC3T3-E1 Cells

被引：68

作者：

Li, Manyu ^{[1
]}

Wang, Wei ^{[1
]}

Wang, Ping ^{[1
]}

Yang, Kun ^{[1
]}

Sun, Hui ^{[1
]}

Wang, Xijun ^{[1
]}

机构：

[1] Heilongjiang Univ Chinese Med, Natl TCM Key Lab Serum Pharmacochem, Harbin 150040, Peoples R China

来源：

MOLECULES | 2010年 / 15卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Liuweidihuang Wan; morroniside; loganin; osteoporosis; MC3T3-E1; OSTEOBLAST DIFFERENTIATION; BONE-FORMATION; APOPTOSIS; OSTEOPOROSIS; EPIDEMIOLOGY; PREVENTION; FAMILY;

D O I：

10.3390/molecules15107403

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Liuweidihuang Wan (LW), initially a well-known formula for curing "wu chi wu ruan", is commonly used nowadays for clinical treatment of Postmenopausal Osteoporosis (PO), but the identity of the effective substance(s) remains unclear. The present study was designed to evaluate the effects of morroniside and loganin isolated from LW on the proliferation, differentiation and apoptosis of MC3T3-E1 cells, as well as the possible mechanism of action. Morroniside and loganin had no effects on the proliferation of MC3T3-E1 cells, but both susbtances could improve the activity of alkaline phosphatase (ALP), and increase the contents of collagen type I and osteocalcin. Simultaneously, the mRNA expression of caspase-3, capase-9, RANKL was down-regulated and that of bcl-2 was up-regulated, which partially explains the anti-osteoporosis mechanism in MC3T3-E1 cells. In conclusion, morroniside and loganin may directly promote the differentiation and inhibit the apoptosis of MC3T3-E1 cells, and accordingly indirectly reduce bone resorption, which makes them promising natural drugs leads for treating PO in the near future.

引用

页码：7403 / 7414

页数：12

共 38 条

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