Effect of Different Forms of Anionic Nanoclays on Cytotoxicity

被引:40
作者
Baek, Miri [3 ]
Kim, In-Sun [3 ]
Yu, Jin [3 ]
Chung, Hae Eun [3 ]
Choy, Jin-Ho [1 ,2 ]
Choi, Soo-Jin [3 ]
机构
[1] Ewha Womans Univ, Ctr Intelligent NanoBio Mat, Div Nano Sci BK21, Dept Bioinspired Sci, Seoul 120750, South Korea
[2] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
[3] Seoul Womens Univ, Dept Food Sci & Technol, Seoul 139774, South Korea
关键词
Anionic Nanoclays; Carbonate Form; Chloride Form; Stability; Cytotoxicity; CLATHRIN-MEDIATED ENDOCYTOSIS; LAYERED DOUBLE HYDROXIDE; INORGANIC NANOPARTICLES; CELLULAR UPTAKE; TOXICITY; SAFETY; CELLS;
D O I
10.1166/jnn.2011.3408
中图分类号
O6 [化学];
学科分类号
070301 [无机化学];
摘要
Anionic nanoclays, so-called layered double hydroxide (LDH) nanoparticles, have been extensively applied as drug delivery systems, since they efficiently enter cells via endocytosis pathway and possess controlled release property. However, the stability of LDHs varies, depending on the type of interlayer anions, which can also affect their toxicity. In this study, we investigated the effects of two different forms of LDH, carbonate form (MgAl-LDH-CO3) and chloride form (MgAl-LDH-Cl), on cytotoxicity in human lung epithelial cells. The result showed that MgAl-LDH-Cl was more easily dissolved into metal ions under simulated lysosomal (pH 4.5) and body fluid (pH 7.4) conditions than did MgAl-LDH-CO3. According to cytotoxicity evaluation, MgAl-LDH-CO3 exhibited high toxicity compared with MgAl-LDH-Cl in terms of induction of oxidative stress, apoptosis and membrane damage. These results suggest that easily dissoluble MgAl-LDH-Cl has low cytotoxicity, while high stability of MgAl-LDH-CO3 is correlated to elevated cytotoxicity.
引用
收藏
页码:1803 / 1806
页数:4
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