DNA fragmentation induced by a cytoplasmic extract from irradiated cells

被引:7
作者
Kurihara, H
Torigoe, S
Omura, M
Saito, K
Kurihara, M
Matsubara, S
机构
[1] Yokohama City Univ, Sch Med, Dept Radiol, Kanazawa Ku, Yokohama, Kanagawa 236, Japan
[2] Yokohama City Univ, Sch Med, Dept Otorhinolaryngol, Kanazawa Ku, Yokohama, Kanagawa 236, Japan
关键词
D O I
10.2307/3579975
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Apoptosis is a mode of cell death characterized by distinct morphological features and DNA fragmentation. The program that leads to apoptosis has been considered to be predominantly extranuclear, and a signal transduction pathway to the nucleus exists during apoptosis, while characteristic events occur in the nucleus. As for radiation-induced apoptosis, the signal transduction pathway remains unclear, especially the sites where the primary effect of radiation occurs. In this study, we demonstrate that a cytoplasmic extract prepared from irradiated cells has the ability to cause DNA fragmentation and that caspase-3 is activated in this extract. Normal nuclei of HeLa S3 cells were added to a cytoplasmic extract made from HL60 cells which had been irradiated with 30 Gy of Cs-137 gamma rays and were incubated. Agarose gel electrophoresis of the added nuclei showed a characteristic DNA laddering pattern. This reaction was blocked by a caspase-3 inhibitor but not by an ICE inhibitor. These observations suggest that a signal transduction pathway from an unknown target of gamma radiation may exist upstream of caspase-3 during radiation-induced apoptosis, (C) 1998 by Radiation Research Society.
引用
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页码:269 / 274
页数:6
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