Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae)

被引:99
作者
Barbieri, E
Paster, BJ
Hughes, D
Zurek, L
Moser, DP
Teske, A
Sogin, ML
机构
[1] Marine Biol Lab, Josephine Bay Paul Ctr Comparat Mol Biol & Evolut, Woods Hole, MA 02543 USA
[2] Marine Biol Lab, Microbiol Divers Course Lab, Woods Hole, MA 02543 USA
[3] Woods Hole Oceanog Inst, Dept Biol, Redfield Lab, Woods Hole, MA 02543 USA
关键词
D O I
10.1046/j.1462-2920.2001.00172.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sexually mature female squid Loligo pealei harbour dense bacterial communities in their accessory nidamental glands (ANGs) and in their egg capsules (ECs). This study describes a molecular approach using the 16S rRNA gene (rDNA) to identify bacterial populations within the ANG and the ECs of the North Atlantic squid species L. pealei. Fluorescent in situ hybridization (FISH) and 16S rDNA analysis showed that predominantly alpha- and, to a lesser extent, gamma-proteobacteria were the predominant components of the ANG and EC bacterial communities. Sequencing results showed the presence of alpha-proteobacterial populations affiliated with the Roseobacter group and additional deep-branching alpha-proteobacterial lineages. In contrast, isolates from the ANG and ECs contained only a few alpha-proteobacteria of the Roseobacter group compared with several gamma-proteobacterial isolates, mostly Shewanella and Pseudoalteromonas species. Most of the ANG-associated bacterial populations were also found within the ECs of L. pealei. The molecular approach allowed the visualization of alpha-proteobacteria as major constituents of a bacterial symbiosis within the reproductive system of the Loliginidae.
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页码:151 / 167
页数:17
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