Arabinoxylan biosynthesis:: Identification and partial characterization of β-1,4-xylosyltransferase from wheat

被引：27

作者：

Porchia, AC ^{[1
]}

Scheller, HV ^{[1
]}

机构：

[1] Royal Vet & Agr Univ, Dept Plant Biol, Plant Biochem Lab, DK-1871 Frederiksberg C, Denmark

来源：

PHYSIOLOGIA PLANTARUM | 2000年 / 110卷 / 03期

关键词：

D O I：

10.1034/j.1399-3054.2000.1100309.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The biosynthesis of arabinoxylan was investigated using microsomal membranes isolated from wheat seedlings. Incubation of the microsomes with UDP-[C-14]-D-xylose resulted in incorporation of radioactivity into polymeric product. Incorporation reached a maximum at day 3 during development of the seedlings. Treatment of the radiolabeled product with Aspergillus niger xylanase A released about 70% of the radioactivity to a 65% ethanol-soluble fraction. The released radioactivity was shown to be in the form of xylobiose, xylotriose and xylotetraose, which are the expected hydrolysis products of endo-xylanases. The synthesized xylan had an apparent molecular mass larger than 500 kDa.

引用

页码：350 / 356

页数：7

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