A cerebrospinal fluid microRNA signature as biomarker for glioblastoma

被引：113

作者：

Akers, Johnny C. ^{[1
]}

Hua, Wei ^{[2
]}

Li, Hongying ^{[3
]}

Ramakrishnan, Valya ^{[1
]}

Yang, Zixiao ^{[2
]}

Quan, Kai ^{[2
]}

Zhu, Wei ^{[2
]}

Li, Jie ^{[1
]}

Figueroa, Javier ^{[1
]}

Hirshman, Brian R. ^{[1
]}

Miller, Brittney ^{[1
]}

Piccioni, David ^{[4
]}

Ringel, Florian ^{[5
]}

Komotar, Ricardo ^{[6
]}

Messer, Karen ^{[3
]}

Galasko, Douglas R. ^{[7
]}

Hochberg, Fred ^{[1
]}

Mao, Ying ^{[7
,8
]}

Carter, Bob S. ^{[1
]}

Chen, Clark C. ^{[1
]}

机构：

[1] Univ Calif San Diego, Ctr Theoret & Appl Neurooncol, San Diego, CA 92103 USA

[2] Fudan Univ, Huashan Hosp, Dept Neurosurg, Shanghai, Peoples R China

[3] UC San Diego Hlth Syst, Moores Canc Ctr, Biostat Dept, La Jolla, CA USA

[4] UC San Diego Hlth Syst, Moores Canc Ctr, Dept Neurosurg, La Jolla, CA USA

[5] Tech Univ Munich, Klinikum Rechts Isar, Dept Neurosurg, Munich, Germany

[6] Univ Miami, Miller Sch Med, Dept Neurol Surg, Miami, FL 33136 USA

[7] Univ Calif San Diego, Dept Neurosci, San Diego, CA 92103 USA

[8] Fudan Univ, Inst Brain Sci, Collaborat Innovat Ctr Brain Sci, State Key Lab Med Neurobiol, Shanghai, Peoples R China

来源：

ONCOTARGET | 2017年 / 8卷 / 40期

关键词：

extracellular vesicle; CSF; liquid biopsy; EXOSOMES; BIOPSY; DIAGNOSIS; RESECTION; MIRNAS; LUMBAR; BRAIN; SET;

D O I：

10.18632/oncotarget.18332

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Purpose: To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. Experimental Design: Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray (R) Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts. Results: miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The " signature" correlated with glioblastoma tumor volume (p=0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF. Conclusion: We report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma.

引用

页码：68769 / 68779

页数：11

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