Digital imaging microscopy of living cells

被引：71

作者：

Rizzuto, R

Carrington, W

Tuft, RA

机构：

[1] Univ Padua, Ctr Study Biomembranes, Dept Biomed Sci, I-37121 Padua, Italy

[2] Univ Padua, CNR, Ctr Study Biomembranes, I-37121 Padua, Italy

[3] Univ Massachusetts, Med Ctr, Biomed Imaging Grp, Worcester, MA 01605 USA

来源：

TRENDS IN CELL BIOLOGY | 1998年 / 8卷 / 07期

关键词：

D O I：

10.1016/S0962-8924(98)01301-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing rise in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.

引用

页码：288 / 292

页数：5

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