Age-associated differences in neutrophil oxidative burst (chemiluminescence)

被引:24
作者
Braga, PC
Sala, MT
Dal Sasso, M
Pecile, A
Annoni, G
Vergani, C
机构
[1] Univ Milan, Dept Pharmacol, Sch Med, IRCCS,Hosp Maggiore, I-20129 Milan, Italy
[2] Univ Milan, Sch Med, IRCCS, Maggiore Hosp,Dept Geriatr, I-20129 Milan, Italy
关键词
oxidative burst; luminol-amplified chemiluminescence; neutrophils; elderly; Candida albicans; zymosan; fMLP; PMA; liquoid;
D O I
10.1016/S0531-5565(98)00012-6
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
Phagocytic defensive functions consist of a sequence of events, including migration, phagocytosis, secretion, and the release of reactive oxygen species (ROS). The last of these (also called "oxidative burst") has not received due attention in the elderly, even though it can be considered the most important event in the process of killing an invading microorganism. The aim of the present study was to investigate the oxidative burst activity of polymorphonuclear neutrophil leukocytes (PMNs) in relation to age, using a technique that specifically identifies ROS production: luminol-amplified chemiluminescence (LACL). Besides the use of LACL, a particular feature of the study was the use of five rather than just one or two different stimulants: two particulate (Candida albicans and zymosan) and three soluble ones [N-formyl-methionyl-leucyl-phenylalanine (fMLP), phorbol 12 myristate 13 acetate (PMA), and polyanetholesulfonate (liquoid)]. This approach allowed us to observe a dichotomy between the effects of Candida and zymosan (particulates), which were not significantly different in the elderly subjects compared to the young controls, and those of fMLP, PMA, and liquoid (solubles), which showed a significant reduction in LACL in the elderly group. Considering the different results obtained with the various stimulants adopted that are all believed to have NADPH oxidase as a common final target of oxidative burst, it may be postulated that aging can influence the different transductional pathways in different ways. (C) 1998 Elsevier Science Inc.
引用
收藏
页码:477 / 484
页数:8
相关论文
共 36 条
[1]  
[Anonymous], 1991, INTRO CENTRIFUGATION
[2]  
BOYUM A, 1991, BLOOD SEPARATION PLA, P217
[3]   Influence of age on oxidative bursts (chemiluminescence) of polymorphonuclear neutrophil leukocytes [J].
Braga, PC ;
Sala, MT ;
Dal Sasso, M ;
Mancini, L ;
Sandrini, MC ;
Annoni, G .
GERONTOLOGY, 1998, 44 (04) :192-197
[4]   INFLUENCE OF ANTIBIOTICS ON FORMYLMETHIONYL-LEUCYL-PHENYLALANINE-INDUCED LEUKOCYTE CHEMILUMINESCENCE [J].
BRIHEIM, G ;
DAHLGREN, C .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (05) :763-767
[5]  
CURNUTTE JT, 1987, J BIOL CHEM, V262, P6450
[6]   AGE-RELATED-CHANGES IN MEMBRANE-RECEPTOR INTERACTIONS [J].
DAX, EM .
ENDOCRINOLOGY AND METABOLISM CLINICS OF NORTH AMERICA, 1987, 16 (04) :947-963
[7]   AGE-RELATED IMPAIRMENT IN PHOSPHATIDYLINOSITOL BREAKDOWN OF POLYMORPHONUCLEAR GRANULOCYTES [J].
FULOP, T ;
VARGA, Z ;
CSONGOR, J ;
FORIS, G ;
LEOVEY, A .
FEBS LETTERS, 1989, 245 (1-2) :249-252
[8]   AGE-DEPENDENT CHANGES IN TRANSMEMBRANE SIGNALING - IDENTIFICATION OF G-PROTEINS IN HUMAN-LYMPHOCYTES AND POLYMORPHONUCLEAR LEUKOCYTES [J].
FULOP, T ;
BARABAS, G ;
VARGA, Z ;
JOZSEF, C ;
CSABINA, S ;
SZUCS, S ;
SERES, I ;
SZIKSZAY, E ;
JENEY, Z ;
PENYIGE, A .
CELLULAR SIGNALLING, 1993, 5 (05) :593-603
[9]  
FULOP T, 1985, CLIN EXP IMMUNOL, V61, P425
[10]   ROLE OF LEUKOCYTE FACTORS AND CATIONIC POLY-ELECTROLYTES IN PHAGOCYTOSIS OF GROUP-A STREPTOCOCCI AND CANDIDA-ALBICANS BY NEUTROPHILS, MACROPHAGES, FIBROBLASTS AND EPITHELIAL-CELLS - MODULATION BY ANIONIC POLY-ELECTROLYTES IN RELATION TO PATHOGENESIS OF CHRONIC INFLAMMATIONAN [J].
GINSBURG, I ;
SELA, MN ;
MORAG, A ;
RAVID, Z ;
DUCHAN, Z ;
FERNE, M ;
RABINOWITZBERGNER, S ;
THOMAS, PP ;
DAVIES, P ;
NICCOLS, J ;
HUMES, J ;
BONNEY, R .
INFLAMMATION, 1981, 5 (04) :289-312