Interface for direct and continuous sample-matrix deposition onto a MALDI probe for polymer analysis by thermal field flow fractionation and off-line MALDI-MS

被引：14

作者：

Basile, F ^{[1
]}

Kassalainen, GE ^{[1
]}

Williams, SKR ^{[1
]}

机构：

[1] Colorado Sch Mines, Dept Chem, Golden, CO 80401 USA

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 09期

关键词：

D O I：

10.1021/ac048391d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple interface based on an oscillating capillary nebulizer (OCN) is described for direct deposition of eluate from a thermal field-flow fractionation (ThFFF) system onto a matrix-assisted laser desorption/ionization (MAL-DI) probe. In this study, the polymer-containing eluent from the ThFFF system was mixed on-line with MALDI matrix solution and deposited directly onto a moving MALDI probe. The result was a continuous sample track representative of the fractionation process. Subsequent off-line MALDI-mass spectrometry analysis was performed in automated and manual modes. Polystyrene samples of broad polydispersity were used to characterize the overall system performance. The OCN interface is easy to build and operate without the use of heaters or high voltages and is compatible with any MALDI probe format.

引用

页码：3008 / 3012

页数：5

共 40 条

[1] Mass discrimination effects in an ion detector and other causes for shifts in polymer mass distributions measured by matrix-assisted laser desorption ionization time-of-flight mass spectrometry [J].