Isolation and characterization of collagen from bigeye snapper (Priacanthus macracanthus) skin

被引：89

作者：

Jongjareonrak, A

Benjakul, S ^{[1
]}

Visessanguan, W

Tanaka, M

机构：

[1] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Thailand

[2] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Klongluang 12120, Phathumthani, Thailand

[3] Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Tokyo 1088477, Japan

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2005年 / 85卷 / 07期

关键词：

collagen; bigeye snapper; skin; acid-solubilized collagen; pepsin-solubilized collagen; solubility;

D O I：

10.1002/jsfa.2072

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were isolated from the skin of bigeye snapper (Priacanthus macracanthus) with yields of 64 and 11 g kg(-1) wet weight, respectively. Both ASC and PSC were characterized as type I collagens with no disulfide bonds. Peptide maps of ASC and PSC digested by V8 protease and lysyl endopeptidase showed some differences in peptide patterns and were totally different from those of calf skin collagen. The maximum solubility was observed at pH 4 and 5 for ASC and PSC, respectively. A sharp decrease in solubility of both collagens in acetic acid was found with NaCl concentration above 30g l(-1). Thermal transitions of ASC and PSC in deionized water were observed with T-max of 30.37 and 30.87 degrees C, respectively, and were lowered in the presence of acetic acid (0.05 mol kg(-1) solution). Therefore, ASC was a major fraction in bigeye snapper skin and it exhibited some different characteristics to PSC. (c) 2005 Society of Chemical Industry.

引用

页码：1203 / 1210

页数：8

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