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Characterization of genetic differences between Mycobacterium avium subsp paratuberculosis type I and type II isolates

被引：45
作者：
Dohmann, K
Strommenger, B
Stevenson, K
de Juan, L
Stratmann, J
Kapur, V
Bulls, TJ
Gerlach, GF
机构：
[1] Hannover Sch Vet Med, Dept Infect Dis, Inst Microbiol, D-30173 Hannover, Germany
[2] Moredun Res Inst, Int Res Ctr, Penicuik EH26 0PZ, Midlothian, Scotland
[3] St George Hosp, Sch Med, Dept Surg, London SW17 0RE, England
[4] Univ Complutense Madrid, Dept Patol Anim 1 Sanidad Anim, Fac Vet, E-28040 Madrid, Spain
[5] Univ Minnesota, Biomed Genom Ctr, St Paul, MN 55108 USA
[6] Univ Minnesota, Dept Microbiol, St Paul, MN 55108 USA
[7] Univ Minnesota, Dept Vet Pathobiol, St Paul, MN 55108 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 11期
关键词：
D O I：
10.1128/JCM.41.11.5215-5223.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A combination of representational difference analysis and comparative DNA sequencing revealed that four type I (sheep) isolates of Mycobacterium avium subsp. paratuberculosis were differentiated from nine type II (bovine) isolates by the presence of an 11-bp insertion in a novel M. avium subsp. paratuberculosis-specific region of genomic DNA. Further, our studies show that M. avium subsp. paratuberculosis type I isolates contain three type-specific loci that are missing in M. avium subsp. paratuberculosis type II but are present in M. avium subsp. avium. Taken together, the results are consistent with the hypothesis that M. avium subsp. paratuberculosis type I strains are an evolutionary intermediate between M. avium subsp. avium and M. avium subsp. paratuberculosis type II isolates or share a subset of M. avium subsp. avium type-specific loci through horizontal transfer.
引用
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页码：5215 / 5223
页数：9
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