No association between the -308 polymorphism in the tumour necrosis factor α (TNFα) promoter region and polycystic ovaries

被引:45
作者
Milner, CR [1 ]
Craig, JE [1 ]
Hussey, ND [1 ]
Norman, RJ [1 ]
机构
[1] Univ Adelaide, Queen Elizabeth Hosp, Reprod Med Unit, Woodville, SA 5011, Australia
关键词
polycystic ovary syndrome; polymorphism; tumour necrosis factor alpha;
D O I
10.1093/molehr/5.1.5
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The tumour necrosis factor (TNF)2 allele appears to be linked with increased insulin resistance and obesity, conditions often found in overweight patients with polycystic ovary syndrome (PCOS). The significance of TNF alpha polymorphism in relation to the clinical and biochemical parameters associated with PCOS was investigated in 122 well-characterized patients with polycystic ovaries (PCO). Of these, 84 had an abnormal menstrual cycle and were classified as having PCOS, while the remaining 38 had a normal menstrual cycle and were classified as having PCO. There were a further 28 individuals without PCO (non-PCO) and 108 individuals whose PCO status was undetermined (reference population). The promoter region of the TNF alpha gene was amplified by polymerase chain reaction (PCR), and the presence or absence of the polymorphism at -308 was determined by single-strand conformational polymorphism (SSCP) analysis. The less common TNF allele (TNF2) was found as TNF1/2 or TNF2/2 in 11/38 (29%) of PCO subjects, 25/84 (30%) of PCOS subjects, 7/28 (25%) of non-PCO subjects, and 45/108 (42%) of the reference population. There was no significant difference in the incidence of the TNF2 allele between the groups. The relationship of TNF genotype to clinical and biochemical parameters was examined. In both the PCO group and the PCOS group, the presence of the TNF2 allele was significantly associated with lower glucose values obtained from the glucose tolerance testing (P < 0.05). The TNF genotype was not significantly associated with any clinical or biochemical parameter measured in the PCO, PCOS or non-PCOS groups. Thus, the TNF alpha -308 polymorphism does not appear to strongly influence genetic susceptibility to polycystic ovaries.
引用
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页码:5 / 9
页数:5
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