Thiol-dependent degradation of protoporphyrin IX by plant peroxidases

被引:21
作者
Dayan, FE
Rimando, AM
Duke, SO
Jacobs, NJ
机构
[1] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA
[2] Dartmouth Coll, Sch Med, Dept Microbiol, Hanover, NH 03755 USA
关键词
porphyrin; tetrapyrrole; degradation; breakdown; turn-over;
D O I
10.1016/S0014-5793(99)00065-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protoporphyrin IX (PP) is the last porphyrin intermediate in common between heme and chlorophyll biosynthesis. This pigment normally does not accumulate in plants because its highly photodynamic nature makes it toxic. While the steps leading to heme and chlorophylls are well characterized, relatively little is known of the metabolic fate of excess PP in plants, We have discovered that plant peroxidases can rapidly degrade this pigment in the presence of thiol-containing substrates such as glutathione and cysteine, This thiol-dependent degradation of PP by horseradish peroxidase consumes oxygen and is inhibited by ascorbic acid. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:227 / 230
页数:4
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