The presence of a pseudogene may affect the use of HPRT as an endogenous mRNA control in RT-PCR

被引：28

作者：

Sellner, LN

Turbett, GR

机构：

[1] Department of Pathology, Royal Perth Hospital, Perth, WA 6000, Wellington St.

来源：

MOLECULAR AND CELLULAR PROBES | 1996年 / 10卷 / 06期

关键词：

semi-quantitative; RT-PCR; HPRT; pseudogene;

D O I：

10.1006/mcpr.1996.0068

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) has been used extensively as a tool to measure expression levels of mRNA species. Many commonly used endogenous mRNA control species are known to have genomic pseudogenes, which can confound RT-PCR results if not accounted for. The hypoxanthine phosphoribosyltransferase gene (HPRT) has previously been used as an mRNA control to circumvent these difficulties, since it was believed that no pseudogenes existed. The existence of a pseudogene of HPRT is reported, and researchers are warned that this gene cannot be used as an endogenous mRNA control without taking appropriate precautions. (C) 1996 Academic Press Limited

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收藏

页码：481 / 483

页数：3

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