Recovery of poly-3-hydroxybutyrate from Alcaligenes eutrophus by surfactant-chelate aqueous system

被引：28

作者：

Chen, YG ^{[1
]}

Chen, J ^{[1
]}

Yu, CR ^{[1
]}

Du, GC ^{[1
]}

Lun, SY ^{[1
]}

机构：

[1] Wuxi Univ Light Ind, Sch Biotechnol, Environm Biotechnol Lab, Wuxi 214036, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 1999年 / 34卷 / 02期

关键词：

poly(3-hydroxybutyrate); recover; surfactant; chelate; aqueous solution; Alcaligenes eutrophus;

D O I：

10.1016/S0032-9592(98)00082-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the recovery of poly(3-hydroxybutyrate) (PHB) from Alcaligenes eutrophus was reported. This process involved the use of a surfactant-chelate aqueous solution. The key factors that influenced the purity, recovery rate and My of recovered PHB were investigated. The purity and recovery rate were determined by the amount of surfactant, the ratio of chelate to dry biomass, pH value, temperature and treatment time, whereas the My was affected by pH value and temperature. The optimal recovery conditions were a 0.12:1 surfactant-to-dry biomass ratio, a 0.08:1 chelate-to-dry biomass ratio, a pH value of 13, a 50 degrees C temperature and a 10-min treatment time. Under such conditions, a purity of 98.7%, a recovery rate of 93.3% and a My of 316000 were obtained. The original My was 402000. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：153 / 157

页数：5

共 16 条

[1] SOLUTION PROPERTIES OF POLY(D-BETA-HYDROXYBUTYRATE) .1. BIOSYNTHESIS AND CHARACTERIZATION [J].

AKITA, S ;

EINAGA, Y ;

MIYAKI, Y ;

FUJITA, H .

MACROMOLECULES, 1976, 9 (05) :774-780

[2]

BERGER E, 1989, Biotechnology Techniques, V3, P227, DOI 10.1007/BF01876053

[3] RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS [J].

BRAUNEGG, G ;

SONNLEITNER, B ;

LAFFERTY, RM .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 6 (01) :29-37

[4]

Burton K., 1968, Methods in Enzymology XIIB, V12, P163

[5]

Doi Y, 1990, MICROBIAL POLYESTER

[6] OPTIMIZATION OF MICROBIAL POLY(3-HYDROXYBUTYRATE) RECOVERY USING DISPERSIONS OF SODIUM-HYPOCHLORITE SOLUTION AND CHLOROFORM [J].

HAHN, SK ;

CHANG, YK ;

KIM, BS ;

CHANG, HN .

BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (02) :256-261

[7]

Holmes P. A., 1990, US Patent, Patent No. [4910145, 4,910,145]

[8] SURFACTANT EFFECTS ON YEAST-CELLS [J].

KING, AT ;

DAVEY, MR ;

MELLOR, IR ;

MULLIGAN, BJ ;

LOWE, KC .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (02) :148-153

[9]

Lee SY, 1996, BIOTECHNOL BIOENG, V49, P1

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 →