Dual-colour imaging with GFP variants

被引：108

作者：

Ellenberg, J ^{[1
]}

Lippincott-Schwartz, J ^{[1
]}

Presley, JF ^{[1
]}

机构：

[1] Inst Child Hlth & Human Dev, Cell Biol & Metab Branch, Bethesda, MD 20892 USA

来源：

TRENDS IN CELL BIOLOGY | 1999年 / 9卷 / 02期

关键词：

D O I：

10.1016/S0962-8924(98)01420-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Green fluorescent protein(GFP) has become an important tool in cell biology and is widely used as a reporter far imaging intracellular proteins and structures in live cells. Recently, spectral variants of GFP with red- and blue-shifted fluorescence emissions have been characterized, opening the possibility of double labelling with two different-coloured GFP fusion proteins. This article reviews recent advances in this technique, with special emphasis on time-lapse imaging applications in living cells.

引用

页码：52 / 56

页数：5

共 31 条

[1] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION
CHALFIE, M
TU, Y
EUSKIRCHEN, G
WARD, WW
PRASHER, DC
[J]. SCIENCE, 1994, 263 (5148) : 802 - 805
[2] Nuclear membrane dynamics and reassembly in living cells: Targeting of an inner nuclear membrane protein in interphase and mitosis
Ellenberg, J
Siggia, ED
Moreira, JE
Smith, CL
Presley, JF
Worman, HJ
LippincottSchwartz, J
[J]. JOURNAL OF CELL BIOLOGY, 1997, 138 (06) : 1193 - 1206
[3] Two-color green fluorescent protein time-lapse imaging
Ellenberg, J
Lippincott-Schwartz, J
Presley, JF
[J]. BIOTECHNIQUES, 1998, 25 (05) : 838 - +
[4] Rapid actin-based plasticity in dendritic spines
Fischer, M
Kaech, S
Knutti, D
Matus, A
[J]. NEURON, 1998, 20 (05) : 847 - 854
[5] GHOSH RN, 1994, J CELL SCI, V107, P2177
[6] Specific covalent labeling of recombinant protein molecules inside live cells
Griffin, BA
Adams, SR
Tsien, RY
[J]. SCIENCE, 1998, 281 (5374) : 269 - 272
[7] WAVELENGTH MUTATIONS AND POSTTRANSLATIONAL AUTOXIDATION OF GREEN FLUORESCENT PROTEIN
HEIM, R
PRASHER, DC
TSIEN, RY
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) : 12501 - 12504
[8] IMPROVED GREEN FLUORESCENCE
HEIM, R
CUBITT, AB
TSIEN, RY
[J]. NATURE, 1995, 373 (6516) : 663 - 664
[9] Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
Hein, R
Tsien, RY
[J]. CURRENT BIOLOGY, 1996, 6 (02) : 178 - 182
[10] Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells
Kanda, T
Sullivan, KF
Wahl, GM
[J]. CURRENT BIOLOGY, 1998, 8 (07) : 377 - 385

← 1 2 3 4 →