Influenza A virus NEP (NS2 protein) downregulates RNA synthesis of model template RNAs

被引:46
作者
Bullido, R [1 ]
Gómez-Puertas, P [1 ]
Saiz, MJ [1 ]
Portela, A [1 ]
机构
[1] Inst Salud Carlos III, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
关键词
D O I
10.1128/JVI.75.10.4912-4917.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The influenza A virus NEP (NS2) protein is an structural component of the viral particle. To investigate whether this protein has an effect on viral RNA synthesis, we examined the expression of an influenza A virus-like chloramphenicol acetyltransferase (CAT) RNA in cells synthesizing the four influenza A virus core proteins (nucleoprotein, PB1, PB2, and PA) and NEP from recombinant plasmids, Influenza A virus NEP inhibited drastically, and in a dose-dependent manner, the level of CAT expression mediated by the recombinant influenza A virus polymerase. This inhibitory effect was not observed in an analogous artificial system in which expression of a synthetic CAT RNA is mediated by the core proteins of an influenza B virus. This result ruled out the possibility that inhibition of reporter gene expression was due to a general toxic effect induced by NEP, Analysis of the virus-specific RNA species that accumulated in cells expressing the type A recombinant core proteins and NEP showed that there was an important reduction in the levels of minireplicon-derived vRNA, cRNA, and mRNA molecules. Taken together, the results obtained suggest a regulatory role for NEP during virus-specific RNA synthesis, and this finding is discussed regarding the biological implications for the virus life cycle.
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页码:4912 / 4917
页数:6
相关论文
共 34 条
[1]   The NS1 protein of human respiratory syncytial virus is a potent inhibitor of minigenome transcription and RNA replication [J].
Atreya, PL ;
Peeples, ME ;
Collins, PL .
JOURNAL OF VIROLOGY, 1998, 72 (02) :1452-1461
[2]   INFLUENZA-B VIRUSES WITH SITE-SPECIFIC MUTATIONS INTRODUCED INTO THE HA GENE [J].
BARCLAY, WS ;
PALESE, P .
JOURNAL OF VIROLOGY, 1995, 69 (02) :1275-1279
[3]   The sendai paramyxovirus accessory C proteins inhibit viral genome amplification in a promoter-specific fashion [J].
Cadd, T ;
Garcin, D ;
Tapparel, C ;
Itoh, M ;
Homma, M ;
Roux, L ;
Curran, J ;
Kolakofsky, D .
JOURNAL OF VIROLOGY, 1996, 70 (08) :5067-5074
[4]   Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus [J].
Collins, PL ;
Hill, MG ;
Cristina, J ;
Grosfeld, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (01) :81-85
[5]   MOLECULAR-CLONING AND SEQUENCING OF INFLUENZA-VIRUS A/VICTORIA/3/75 POLYMERASE GENES - SEQUENCE EVOLUTION AND PREDICTION OF POSSIBLE FUNCTIONAL DOMAINS [J].
DELALUNA, S ;
MARTINEZ, C ;
ORTIN, J .
VIRUS RESEARCH, 1989, 13 (02) :143-156
[6]   INFLUENZA-VIRUS NS1 PROTEIN STIMULATES TRANSLATION OF THE M1 PROTEIN [J].
ENAMI, K ;
SATO, TA ;
NAKADA, S ;
ENAMI, M .
JOURNAL OF VIROLOGY, 1994, 68 (03) :1432-1437
[7]   EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].
FUERST, TR ;
NILES, EG ;
STUDIER, FW ;
MOSS, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126
[8]  
Gómez-Puertas P, 1999, J GEN VIROL, V80, P1635, DOI 10.1099/0022-1317-80-7-1635
[9]   Influenza virus matrix protein is the major driving force in virus budding [J].
Gómez-Puertas, P ;
Albo, C ;
Pérez-Pastrana, E ;
Vivo, A ;
Portela, A .
JOURNAL OF VIROLOGY, 2000, 74 (24) :11538-11547
[10]   EXPRESSION OF INFLUENZA VIRUS-NS2 NONSTRUCTURAL PROTEIN IN BACTERIA AND LOCALIZATION OF NS2 IN INFECTED EUKARYOTIC CELLS [J].
GREENSPAN, D ;
KRYSTAL, M ;
NAKADA, S ;
ARNHEITER, H ;
LYLES, DS ;
PALESE, P .
JOURNAL OF VIROLOGY, 1985, 54 (03) :833-843