DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI

被引:124
作者
Jurica, NS
Monnat, RJ
Stoddard, BL [1 ]
机构
[1] Univ Washington, Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA
[2] Univ Washington, Grad Program Mol & Cell Biol, Seattle, WA 98109 USA
[3] Univ Washington, Dept Pathol, Seattle, WA 98195 USA
关键词
D O I
10.1016/S1097-2765(00)80146-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the LAGLIDADG intron-encoded homing endonuclease I-Crel bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-Crel is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-Crel homodimer, each of which contains a separate bound divalent cation.
引用
收藏
页码:469 / 476
页数:8
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